Covalent cross-linking of proteins without chemical reagents
نویسندگان
چکیده
منابع مشابه
Chemical cross linking versus high energy electron beam cross linking of HDPE: electrical properties study
Cross linking of high density polyethylene (HDPE) was first performed via high energy10 Mev electron beam (EB) irradiation. HDPE was also cross linked withDicumyle peroxide (DCP).The gel content of samples was determined by solvent extraction.Degree of cross linking was evaluated by hot set apparatus; as well .In order to clarify the effectof nature of cross linking, correlation of electrical p...
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Proteases are important enzymes that their role in various industries is undeniable. However, keeping enzymes stable during its activity in harsh conditions is so important. In this study, protease enzyme was immobilized on the porous silica particles and its stability in different temperatures and pHs was evaluated. First silica particles were aminated by 3-aminopropyltriethoxysilane then the ...
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A water-soluble carbodiimide has been used to promote the formation of amide bonds between carboxyl residues on cytochrome b5 and lysyl residues on cytochrome bg reductase. The visible and UV absorption spectrum of the purified cross-linked complex was identical with the sum of the spectra of the individual enzymes, and the average apparent molecular weight of the complex, determined by sodium ...
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proteases are important enzymes that their role in various industries is undeniable. however, keeping enzymes stable during its activity in harsh conditions is so important. in this study, protease enzyme was immobilized on the porous silica particles and its stability in different temperatures and phs was evaluated. first silica particles were aminated by 3-aminopropyltriethoxysilane then the ...
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The reactions of two spin-label cross-linking reagents with components of red blood cells have been studied. A bifunctional bis(N-hydroxysuccinimide ester) reagent passes readily through the membrane of intact red cells and reacts almost exclusively with hemoglobin. The rotational motion of hemoglobin inside of red cells may be studied by electron paramagnetic resonance (EPR) by using this spin...
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ژورنال
عنوان ژورنال: Protein Science
سال: 2002
ISSN: 0961-8368,1469-896X
DOI: 10.1110/ps.4390102